Agrenvec/Easy blot Mini YKit Follistatina – RF0082/RF0082/1-unit-免疫在线蚂蚁淘旗下平台-

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Agrenvec/Easy blot Mini YKit Follistatina – RF0082/RF0082/1-unit

来自 : 发布时间：2025-01-16

Antibody Source:

Rabbit

Clonality:

Polyclonal-IgG

Western Blot:

Suggested starting dilution 1/500. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.

ELISA:

Suggested starting dilution 1/500. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of 0.2-1 ng/well of rhuman Activin A Active (RF0010).

Description: Serologic cualitative detection of rh Activin A using AntiHuman Activin A IgG developed in rabbit and purified by affinity chromatography on protein G (> 98% purity).

Immunogen: Highly pure (>97%) recombinant human Activin A expressed in plants.

Applications:

Western Blot: Suggested starting dilution 1/500. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
ELISA: Suggested starting dilution 1/500. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of 0.2-1 ng/well of rhuman Activin A Active (RF0010).

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Fig 1. Western Blot analysis of rhuman Follistatin using Anti-Human Follistatin IgG (RF0060)

Human Follistatin protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:1,000 of Anti-Human Follistatin IgG. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM; Lane 2: 0.25 ug f non glycosylated rHuman Follistatin protein produced in a prokaryotic expression system. Lane 3: 0.25 ug of glycosylated isoforms of rH Follistatin produced in an eukaryotic expression system.

Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.Optimal dilution conditions should be determined by the final user.Bulk sizes are available upon request. For R&D purposes only.Purchaser must determine the suitability of the product(s) for their particular use.

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发布于： 2025-01-16 阅读（）

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