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Agrenvec/Anti-Human Follistatin (IgG) – RF0060/RF0060/100-microg
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发布时间:2025-01-16
Description:
Rabbit Polyclonal Antibody (IgG) to FollistatinApplication:
WB, ELISAReactivity:
HumanConjugate:
UnconjugatedELISA:
Suggested starting dilution 1/500 anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.Description: Anti-Human Follistatin IgG developed in rabbit and purified by affinity chromatography on protein G (> 98% purity).
Immunogen: Highly pure (>97%) recombinant human Follistatin expressed in plants.
Applications:
- ELISA: Suggested starting dilution 1/500. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of <10 ng/well of rhuman Follistatin (RF0043).
- Western Blot: Suggested starting dilution 1/500 anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
Fig 1. Western Blot analysis of rhuman Follistatin using Anti-Human Follistatin IgG (RF0060)
Human Follistatin protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:1,000 of Anti-Human Follistatin IgG. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM; Lane 2: 0.25 ug f non glycosylated rHuman Follistatin protein produced in a prokaryotic expression system. Lane 3: 0.25 ug of glycosylated isoforms of rH Follistatin produced in an eukaryotic expression system.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.Optimal dilution conditions should be determined by the final user.Bulk sizes are available upon request. For R&D purposes only.Purchaser must determine the suitability of the product(s) for their particular use.本文链接: http://list.immuno-online.com/view-1546329322.html
发布于 : 2025-01-16
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